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LifeTein®, the custom peptide synthesis service company, has developed proprietary PeptideSynTM technology. This technology provides a platform for continuous peptide synthesis using Fmoc and Boc chemistry and a proprietary solid support resin. This is one of the many reasons why LifeTein® can offer competitive custom peptide synthesis prices.
LifeTein routinely uses our proprietary technologies to produce peptides >100 amino acids. The longest peptide we have made to date is 169 amino acids. You will not be charged if we are unable to synthesize your peptide successfully. We promise to provide the HPLC purified peptide with correct molecular weight analyzed by Electrospray ionization (ESI) Mass Spectrometry.
NUCLEIC ACID VECTORS AND USES THEREOF: A peptide of 133 amino acids was synthesized by LifeTein. The peptide sequence includes at least one cell penetrating peptide (CPP) domain, one secretion signal sequence, one DNA binding domain, and other SHT protein.
Phosphorylation: Posttranslational modification on Tyr, Ser and Thr residues, signal transduction, gene expression and protein-protein interaction
Biotinylation: Strong affinity for streptavidin and avidin to be used in histocytochemistry, immunoassays, and fluorescence based flow cytometry
Cyclization: Peptides can be cyclized by disulfide bond, head-to-tail, amide or thioester cyclization to stabilize the peptide conformation, increase bioactivity, and enzyme stability
Long Peptide Synthesis: An alternative approach to exogenous protein expression
Cell Penetrating Peptides: Deliver biologically active cargo to the cell interior; Enter the plasma membrane of a cell independent of a membrane receptor
Linker, Spacer, PEGylations: Reduce steric hindrance at the binding sites of the peptide and improve proteolytic stability and solubility of peptides
Fluorescein: FITC, FAM, TAMRA, Rhodamine, Dansyl, 4-Dinitrophenyl (Dnp), 7-methoxycoumarin acetic acid (Mca) Cy3, Cy5, Cy7 for Fluorescence based assays, flow cytometry, protein-protein interaction, and localization studies
Short Peptide Synthesis: dipeptides or tripeptides as the ligands for signal transduction or cosmetic applications
D Amino Acid Peptides: Resistant to proteases for higher stability and special structure for peptide drug design
TFA Free Acetate Peptides: Low toxic peptides for peptide drug and cell studies
Rush Peptide Service: Receive your peptides in a week and accelerate your research
Peptide Magnetic Agarose Conjugate: Conjugate your peptide to the magnetic agarose for easy binding assay
N Terminal Acetylation: Increase peptide stability by preventing N and C-terminal degradation.
C Terminal Amidation: Neutralize negative charge created by the C-terminal COOH. Mimic native proteins, and remove hydrogen bonding which may interfere with the assays
Multiple Antigenetic Peptides (MAP): Branched peptides MAP4 or MAP8 have a high molar ratio of the peptide antigen for higher immune responses
Immunogenic Peptides: BSA, KLH or OVA Conjugation: Conjugated peptides for ELISA, antibody production applications
Peptide Library: Large scale of peptide screening for new drug candidates
Stapled Peptide and Special Amino Acids: Palmitic acid increases the cell permeability and helps binding of the peptides to cell membrane.
The process of introducing drugs into cells has always proved to be a major challenge for scientists. However, cell-penetrating peptides (CPPs) have the ability to enter a cell through the plasma membrane independent of a membrane receptor. They are usually small peptides 10–30 residues in length.
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The following table shows a selection of currently known CPPs, together with their origins and sequences. Click here for more details.
Incomplete deprotection and amino acid-coupling reactions can complicate certain processes. Longer reaction times and the use of increased strength reagent could solve this problem. However, in some extreme cases, these methods are insufficient and the protecting group cannot be removed efficiently. In addition, the side chains of some sequences render them prone to self-association by hydrogen bonding. This leads to aggregation and the formation of beta-sheet-like secondary structures that can halt peptide synthesis.
LifeTein's optimized protocol and PeptideSyn technology provide a method for circumventing the problems associated with difficult sequences. This technology can change peptide structure by protecting some of the peptide amide bonds, giving rise to peptides that contain tertiary amides at specific intervals. This leads to better preservation of the peptide chain, and more efficient deprotection and coupling reactions.
Case 1: Solid-phase synthesis of a peptide with four phosphorylation sites.
A 14 amino acid peptide (molecular weight: 1981.53) with four phosphorylation sites was synthesized at 95% purity and delivered in 3 weeks: xxxpSpTxxxpSxxxpTx
Case 2: Long peptide synthesis is always difficult and challenging. Recently, a 169-amino-acid peptide was successfully synthesized in 4 weeks. The following case is a very hydrophobic 68 amino acid peptide (85% purity) with a FITC modification at the N terminus. The peptide was successfully synthesized in 4 weeks.
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1. You can use the following table to estimate peptide synthesis price. Please inquire for more pricing information:
|Crude||>85% (Get a quote for desalted, 70%, 75%, 80% and other purities)||>95% (Get a quote for >98%, 99% and other purities)|
(Get a quote for 5-9mg, 10-14mg and other quantities )
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