Peptide Synthesis: bio-inert HPLC system!

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What Is a Bio-Inert HPLC System?

A bio-inert HPLC system is one built so that no sample contacts active metal surfaces within the flow path—typically using PEEK, ceramic, or titanium‑based components, or chemically inert coatings. This eliminates unintended adsorption of peptides, proteins, conjugates, and other biomolecules to stainless-steel surfaces, which can cause loss, peak tailing, carryover, and reduced reproducibility.

Why Bio-Inert Matters for Peptides, Conjugates & Modifications?

• Reduced Adsorption, Improved Recovery: Biomolecules like peptides and phosphopeptides are prone to bind to metal surfaces, reducing recovery and causing tailing. Bio-inert systems eliminate this risk, yielding cleaner peaks and higher analyte recovery.
• Better Sensitivity & Reproducibility: Studies show ~30% increase in peak height and up to ~40% better signal-to-noise for oligonucleotides and peptides versus legacy steel systems. Area RSD dropped from ~6% to < 1% in peptide analyses such as GLP-1 profiles.
• Fast Adoption, No Passivation Needed: Bio-inert systems significantly reduce or eliminate the need for lengthy passivation protocols. Customers can switch methods and run analyses rapidly without primer runs or conditioning.
• Harsh Conditions Tolerated: Exposure to high-salt buffers or extreme pH is common in IEX purification or conjugation workflows. Bio-inert systems sustain these conditions without degradation to the flow path or sample loss.
• Multi-Modal Chromatography Compatibility: Supports RP, IEX, SEC, and peptide mapping, enabling LifeTein’s clients to purify and analyze a broad range of peptide or conjugate samples in a unified platform.

Advantages for LifeTein’s Applications

When LifeTein deploys this bio-inert HPLC system for peptide–protein conjugates, labelled peptides, and other modified conjugates, clients benefit:

• Higher yields & purity: Minimal sample loss during purification and crisp peak shapes.
• Reliable detection of trace impurities: Ability to resolve low-level species in conjugates or labeling reactions with higher signal-to-noise.
• Compatibility with conjugation chemistries: Withstands harsh buffers or pH used during labeling or protein–peptide coupling.
• No cross-contamination: Carryover below 9 ppm ensures one run doesn’t impact the next.
• Higher throughput & faster QC: Ultra‑fast separations reduce runtime, faster method transfer, ideal for R&D and scale-up.

Quotations

• Method 1: Obtain a quote. Please use the online quotation form:

                       

• Method 2: Please complete the Quotation Form for the peptide you would like to purchase and either fax it to 1-888-791-1618 or email it to peptide@lifetein.com. We will send you a quote within 24 hours.