Custom Rabbit Monoclonal Antibody Services 🐇

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Custom Rabbit Monoclonal Antibody Services 🐇

Please click here to get a rabbit monoclonal antibody service quote now!

                        Antibody Service Quote

Rabbit monoclonal antibody services from any immunogens to monoclonal antibodies in four months
  1. Immunize two rabbits with recombinant protein or conjugated peptide/hapten (60 days)
  2. If have the ongoing project at other vendors, please contact us on how to provide the fresh spleen to us for the splenocyte isolation.
  3. Isolate PMBC or splenocytes from selected rabbit (14 days)
  4. Screen and culture antibody-secreting B-cells
  5. Clone variable regions of rabbit lgG genes from identified specific B lymphocytes
  6. Subclone heavy and light chain genes into expression vectors (30 days)
  7. Validate express recombinant rabbit mAb’s blinding to antigen by indirect ELISA
  8. Deliverables: Five clones. Full length heavy & light chain genes in a non-proprietary plasmid. 100ug purified antibodies.
Rabbit Monoclonal Antibodies based on a single cell platform with a shorter screen time

Strong Affinity: The rabbit monoclonal antibodies are 10-100 times higher affinity than mouse monoclonal antibodies.

High Specificity: The rabbit monoclonal antibodies have improved sensitivity for assays with lower false positive signals.

Improved Stability: The rabbit's IgGs have extra disulfide bonds for improved stability and shelf life.

Broader epitope recognition: The rabbit antibodies recognize small molecules or haptens including lipids, hormones and small drugs. The antibodies can be raised for peptides with modifications, the protein with conformational epitopes or enzyme with cleavage sites.

How is the Rabbit Monoclonal Antibody Made?

Rabbit Monoclonal Antibody Service

Please click here to get a rabbit monoclonal antibody service quote now!

                        Antibody Service Quote

Reference:

Jia Shen. et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature 497, 383–387 (16 May 2013), doi:10.1038/nature12080

LifeTein helped designed and synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific antibody productions. The phospo-specific antibodies by LifeTein were confirmed to react with the epidermal growth factor receptor (EGFR). The Hung's lab showed that AGO2-Y393 phosphorylation mediates EGFR-enhanced cell survival and invasiveness under hypoxia. These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells.

... The following peptides were chemically synthesized for antibody production in mice (Lifetein Conc.), Elisa verification (LifeteinConc.) and peptide competition assay in immunohistochemistry (IHC)... Supplementary information