LifeTein® offers gene synthesis service, protein expression in E.coli. and Baculovirus/Insect cell systems. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins. Baculovirus expression system has the advantages of high-level expression and ease of scale-up with posttranslational modifications.
Order your Gene Synthesis Service at competitive price NOW! Email us at email@example.com for a quote.
|<1.5 kb||2-3 weeks||$0.25/bp, Promotion: *$0.18/bp|
|1.5-3 kb||2-4 weeks||$0.25/bp, Promotion: *$0.18/bp|
|3-7 kb||4-6 weeks||Please Inquire|
|>7 kb||7-8 weeks||Please Inquire|
All prices are applied to standard gene sequences only. Minimum charge $150/gene. Starting from $30 for subcloning into any vector of your choice.
Highly discounted price at $0.15/bp for 25 genes or more.
|It is easy. Just email us a gene sequence. We will have the gene designed, optimized, synthesized, and cloned. Or send us the protein sequence and we will send you minigrams of proteins.|
|It is flexible. Your choice of getting synthetic genes, peptides or proteins. If the 6His tag does not give us efficient expression, we will change the 6His tag to GST for free.|
|It is economical. We offer package price from gene synthesis, cloning, protein expression to crystallization or antibody production.|
|It is productive. Optimized refolding methodology is available to solve the protein solubility issues. Enhanced expression vectors are used to increase the protein expression scale and bioactivity.|
|It is large. Need more genes, peptides or proteins? We are able to scale up the gene synthesis, peptide production or protein expression to hundreds of litres or gram levels.|
1. How long does a baculovirus expression project usually take?
Starting with the baculovirus expression plasmid, our service can be completed in 8-9 weeks.
2. What advantages does a baculovirus expression system provide?
Baculovirus expression systems can be used when a target protein with posttranslational modifications is needed for functional and structural studies. While peptide synthesis is usually for difficult proteins that are not feasible within any protein expression system. In addition, the eukaryotic Baculovirus/Insect system is completely safe.
3. What if my gene does not express well?
We have different expression strains and leader sequences for optimizing expression. Options of refolding methods and tags are used for the best conditions.
Recombinant proteins accumulated intracellularly form inclusion bodies, or insoluble aggregates of misfolded protein lacking biological activity. Proteins have to fold in a correct conformation. In this way, specific sites of the right size, shape, and polarity are created for their ligands or receptors. Conventional methods for refolding of insoluble recombinant proteins include slow dialysis, ion exchange, gel filtration, or dilution into a buffer of near neutral pH.
LifeTein's refolding technology is based on a simple and historically successful protocol. This method reduces protein waste and minimizes unnecessary analysis. The simplified protocol provides reliable, high-yield and high concentration of refolded proteins.
Quotations: Email your gene synthesis service or protein service inquiry to Dr. Yu Cao firstname.lastname@example.org. We will send you a formal quote within 24 hours.