Gene Synthesis Service, plasmid preparation services,Recombinant Protein Expression Service: Protein Expression in Bacterial and mammalian transient cell system

Tip: How to detect small peptide clearly and sensitively by Western blotting or SDS-PAGE?

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LifeTein^® offers gene synthesis service, plasmid preparation service, protein expression in E.coli. and mammalian transient protein expression service. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins.

LifeTein's plasmid preparation services, at our lab facility in the United States, provide high-quality plasmids from Mini to Giga scale and endotoxin-free service with a reliable and fast turnaround time of 2-4 business days. For example, our Giga Prep service isolates 20 mg of ultrapure plasmid DNA. The delivery of 10 mg for high-copy plasmid or 4 mg for low-copy plasmid is guaranteed. The bacterial colony, glycerol stock, or plasmid DNA are all acceptable. The endotoxin-free (< 0.1 EU/µg DNA) service is available.

LifeTein’s mammalian protein expression service provides flexible and rapid production of human, other mammalian proteins and recombinant monoclonal antibodies. Mammalian expression systems generate recombinant proteins with unique advantages of correct folding, proper post-translational modifications, and optimal structure or native epitope antigenicity.

In combining with the proprietary codon optimization, gene synthesis, and plasmid preparation, our integrated mammalian expression systems, as well as US-New Jersey based facility, are capable of maximizing the protein productivity with speedy delivery.

LifeTein provides the service from the virtual sequence to purified protein in as little as four weeks. Protein production is supplied at flexible scales and formats, including from 100 ml assay scale up to 20 liters of production in serum-free and suspension culture. You will receive recombinant protein and antibody products that meet your particular specifications and expectation.

LifeTein provides hybridoma sequencing or antibody sequencing for the cDNA encoding the variable domains of heavy (VH) and light (VL) chains of your monoclonal antibody from different species. The engineered antibody genes are constructed into our mammalian cell expression vector for both transient and/or stable cell line antibody production.

Order your Plasmid Preparation Service at a competitive price! Email us at sales@lifetein.com for a quote.

Order your Gene Synthesis Service at a competitive price! Now it is now only $0.18/bp after 30% off. Email us at sales@lifetein.com for a quote.

• Advantages
• Features
• FAQs
• Refolding Tech

	It is easy. Your local access to the plasmid preparation, mammalian protein expression facility in New Jersey, USA. Just email us a gene sequence or plasmids in any form. We will have the gene designed, optimized, synthesized, and cloned. Or send us the protein sequence and we will send you minigrams of proteins. Proteins are shipped in dry ice.
	It is flexible. Your choice of getting synthetic genes, plasmids, peptides or proteins. If the 6His tag does not give us efficient expression, we will change the 6His tag to GST for free.
	It is economical. We offer package price from gene synthesis, plasmid preparation, cloning, protein expression to antibody production.
	It is productive. Optimized refolding methodology is available to solve the protein solubility issues. Enhanced expression vectors are used to increase the protein expression scale and bioactivity.
	It is large. Need more genes, plasmids, peptides or proteins? We are able to scale up the gene synthesis, plasmid preparation, peptide production or protein expression to hundreds of litres or gram levels.

Features

Plasmid Preparation Services: We are committed to delivering high-quality plasmid DNA for cell transfection, transgenic injection, DNA vaccine development, and other applications.

• Giga Prep service: Guarantee 10 mg for high-copy plasmid or 4 mg for low-copy plasmid. 3-5 business days.
• Mega Prep service: Guarantee 3 mg for high-copy plasmid or 1 mg for low-copy plasmid. 3-4 business days.
• Maxi Prep service: Guarantee 1 mg for high-copy plasmid or 500 µg for low-copy plasmid. 2-4 business days.
• Midi Prep service: Guarantee 300 µg for high-copy plasmid or 150 µg for low-copy plasmid. 2-4 business days.
• Mini Prep service: Guarantee 20 µg for high-copy plasmid or 5 µg for low-copy plasmid. 2-4 business days.
• The bacterial colony, glycerol stock, or plasmid DNA are acceptable. Endotoxin-free (< 0.1 EU/µg DNA), very low-level endotoxin (< 1.0 EU/µg DNA), or low-level endotoxin (~9.3 EU/µg DNA) services are available.

Gene Synthesis Services: Subclone to your own vector at competitive price. By default, we clone genes into our pUC19 or pUC57 vector for free, subcloning will add additional two days.

• Free codon optimization: Optimized genes have higher protein expression in their correspondent hosts.
• Fast turnaround time. 1-2 weeks for <1.5 kb genes. 2-3 weeks for genes of 1.5 kb to 5 kb.
• Advanced synthetic technology for complex genes with repetitive, AT or GC rich DNA.

Bacterial Expression System: Different optimized conditions are used for clones in order to find the optimum soluble expression condition with our system. The pET expression system provides high level of protein production.

• E. coli system for soluble protein with high stability
• Classic ploy-Histidine tag for most protein of interest
• High affinity FLAG or GST tag for protein with very low expression level

Mammalian transient protein expression service

• Mammalian transient protein expression system to meet the demand for economic and large amount of eukaryotic proteins in their native conformation
• Different purification tags on customer’s request
• Cell culture in 293 or CHO suspension culture cell lines for optimum expression

Frequently Asked Questions: Protein, antibody expression, and purification

1. How long does a mammalian transient protein expression project usually take?

Starting with the mammalian expression plasmid, our service can be completed in 5-9 weeks.

2. What advantages does a mammalian transient protein expression system provide?

Mammalian expression systems can be used when a target protein with posttranslational modifications is needed for functional and structural studies. While peptide synthesis is usually for difficult proteins that are not feasible within any protein expression system. In addition, the eukaryotic system is completely safe. You just need to provide the protein sequence, antibody sequence or hybridoma cells, we will provide the proteins or antibodies.

3. What if my gene does not express well?

We have different expression strains and leader sequences for optimizing expression. Options of refolding methods and tags are used for the best conditions.

LifeTein Refolding Technology

Recombinant proteins accumulated intracellularly form inclusion bodies, or insoluble aggregates of misfolded protein lacking biological activity. Proteins have to fold in a correct conformation. In this way, specific sites of the right size, shape, and polarity are created for their ligands or receptors. Conventional methods for refolding of insoluble recombinant proteins include slow dialysis, ion exchange, gel filtration, or dilution into a buffer of near neutral pH.

LifeTein's refolding technology is based on a simple and historically successful protocol. This method reduces protein waste and minimizes unnecessary analysis. The simplified protocol provides reliable, high-yield and high concentration of refolded proteins.

Quotations: Email your gene synthesis service, plasmid preparation service, or protein service inquiry to sales@lifetein.com. We will send you a formal quote within 24 hours.