Gene Synthesis Service and Protein Expression Service

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Gene Synthesis Service, Recombinant Protein Expression Service: Protein Expression in Bacterial and Baculovirus Insect System

LifeTein® offers gene synthesis service, protein expression in E.coli. and Baculovirus/Insect cell systems. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins. Baculovirus expression system has the advantages of high-level expression and ease of scale-up with posttranslational modifications.

Order your Gene Synthesis Service at competitive price NOW! Email us at gene@lifetein.com for a quote.

Gene Length

Turnaround

List Price

<1.5 kb 2-3 weeks $0.34/bp
1.5-3 kb 2-4 weeks $0.34/bp
3-7 kb 4-6 weeks Please Inquire
>7 kb 7-8 weeks Please Inquire

All prices are applied to standard gene sequences only. Minimum charge $150/gene.
Highly discounted price at $0.22/bp for 25 genes or more.


Protein Expression Service and Crystallization

Protein Expression in E.coli It is easy. Just email us a gene sequence. We will have the gene designed, optimized, synthesized, and cloned. Or send us the protein sequence and we will send you minigrams of proteins.
Protein Purification It is flexible. Your choice of getting synthetic genes, peptides or proteins. If the 6His tag does not give us efficient expression, we will change the 6His tag to GST for free.
Protein Ligand Binding It is economical. We offer package price from gene synthesis, cloning, protein expression to crystallization or antibody production.
Protein Crystals It is productive. Optimized refolding methodology is available to solve the protein solubility issues. Enhanced expression vectors are used to increase the protein expression scale and bioactivity.
Protein Structure It is large. Need more genes, peptides or proteins? We are able to scale up the gene synthesis, peptide production or protein expression to hundreds of litres or gram levels.

Features

  • Gene Synthesis Services: Subclone to your own vector at competitive price. By default, we clone genes into our pUC19 or pUC57 vector for free, subcloning will add additional two days.
    • Free codon optimization: Optimized genes have higher protein expression in their correspondent hosts.
    • Fast turnaround time. 1-2 weeks for <1.5 kb genes. 2-3 weeks for genes of 1.5 kb to 5 kb.
    • Advanced synthetic technology for complex genes with repetitive, AT or GC rich DNA.
  • Bacterial Expression System: Different optimized conditions are used for clones in order to find the optimum soluble expression condition with our system. The pET expression system provides high level of protein production.
    • E. coli system for soluble protein with high stability
    • Classic ploy-Histidine tag for most protein of interest
    • High affinity FLAG or GST tag for protein with very low expression level
  • Baculovirus Insect Cell Expression System:
    • Insect cell system to meet the demand for economic and large amount of eukaryotic proteins in their native conformation
    • Different purification tags on customer’s request
    • Cell culture in Sf9 or High Five suspension culture cell lines for optimum expression

Frequently Asked Questions: Protein expression, purification and crystallization

1. How long does a baculovirus expression project usually take?

Starting with the baculovirus expression plasmid, our service can be completed in 8-9 weeks.

2. What advantages does a baculovirus expression system provide?

Baculovirus expression systems can be used when a target protein with posttranslational modifications is needed for functional and structural studies. While peptide synthesis is usually for difficult proteins that are not feasible within any protein expression system. In addition, the eukaryotic Baculovirus/Insect system is completely safe.

3. What if my gene does not express well?

We have different expression strains and leader sequences for optimizing expression. Options of refolding methods and tags are used for the best conditions.

LifeTein Refolding Technology

Recombinant proteins accumulated intracellularly form inclusion bodies, or insoluble aggregates of misfolded protein lacking biological activity. Proteins have to fold in a correct conformation. In this way, specific sites of the right size, shape, and polarity are created for their ligands or receptors. Conventional methods for refolding of insoluble recombinant proteins include slow dialysis, ion exchange, gel filtration, or dilution into a buffer of near neutral pH.

LifeTein's refolding technology is based on a simple and historically successful protocol. This method reduces protein waste and minimizes unnecessary analysis. The simplified protocol provides reliable, high-yield and high concentration of refolded proteins.


Quotations: Email your gene synthesis service or protein service inquiry to Dr. Yu Cao yu.cao@lifetein.com. We will send you a formal quote within 24 hours.

Custom Fluorescent Antibody and Protein Labeling
  • We can conjugate your antibody or protein to many labels.
  • Biotin, Strepatvidin, Horseradish peroxidase (HRP) and Alkaline Phosphatase (AP)
  • Fluorescent dyes
Antibody Conjugate Service