Product Name | FLAG-Cysteine, DYKDDDDK-Cysteine |
Product Quantity | 1 mg, 5 mg, 100 mg |
Catalog Number | LT1340 |
Molecular Weight | 1016.13 |
Formula | C44H65N11O21S1 |
Sequence | Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Cys, DYKDDDDKC |
Purity | >95% |
Product Description | The DYKDDDDK-Cysteine peptide (Flag) is used as a fusion tag for immunoaffinity chromatography, which is consisted of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site. It allows elution under non-denaturing conditions. This epitope tag is a short hydrophilic, highly charged peptide. It is the most widely used epitope tag employed in structural and functional studies of proteins. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Order anti-DYKDDDDK monoclonal antibody from here. |
Scientific Background | FLAG-Cysteine, DYKDDDDK-Cysteine is a synthetic research peptide in the FLAG epitope peptide category. The FLAG epitope has the sequence DYKDDDDK and is widely used as a compact antibody-recognition tag. Synthetic FLAG peptide and cysteine-extended derivatives can serve as anti-FLAG binding/competition controls, affinity-elution reagents, immunochemical standards, or conjugation-ready tag peptides. An added cysteine is a chemical handle and is not part of the canonical eight-residue FLAG epitope. |
Research Applications | - anti-FLAG antibody binding and competition assays
- FLAG affinity-elution/control studies
- epitope-tag analytical controls
- cysteine-mediated conjugation studies
|
Experimental Notes | The exact supplied sequence and modification state should be used when interpreting published results. Activity reported for a parent peptide, labeled derivative, formulation, species variant, or related analog should not automatically be assigned to this construct. |
Selected References | - Minimal sequence analysis of the FLAG DYKDDDDK epitope
- FLAG DYKDDDDK and His6 tags used for detection and affinity purification
|
