Product Name | 3X DYKDDDDK Peptide |
Product Quantity | 1 mg, 5 mg, 100 mg, 1 gram |
Catalog Number | LT8153 |
Molecular Weight | 2861.85 |
Formula | C120H169N31O49S |
Sequence | MDYKDHDGDYKDHDIDYKDDDDK, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
|
Purity | >95% |
Product Description | MDYKDHDGDYKDHDIDYKDDDDK is a fusion FLAG tag including an enterokinase-cleavage site. It is designed for immunoaffinity chromatography. The tricosapeptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity columns, especially when a DYKDDDDK-tagged protein is sensitive to low pH. The 3x DYKDDDDK Peptide may interfere with protein-determination assays and with absorbance at 280 nm. The peptide can be removed from the eluate by desalting. See details about the cell penetrating peptides: https://www.lifetein.com/Cell_Penetrating_Peptides.html |
Scientific Background | 3X DYKDDDDK Peptide is a 23-residue synthetic peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. The sequence contains 4 Lys and 0 Arg residues, contributing cationic character; contains 3 aromatic residues that can contribute to hydrophobic or aromatic interactions. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Research Applications | - LC-MS/HPLC analytical method development
- sequence-specific assay controls
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Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
