LifeTein offers a comprehensive range of magnetic beads designed for bioseparation applications across all scales—from microliter volumes to multi-liter preparations. These nano-sized superparamagnetic beads are covalently functionalized with a variety of chemical groups and affinity ligands, including maleimide, primary amine, NHS (N-hydroxysuccinimide), carboxylic acid, streptavidin, protein A, reduced glutathione, nickel-charged NTA, and specialized groups for DNA/RNA purification.
Highlights
| Feature | Iodoacetyl-Activated Beads (Non-Cleavable, Thiol-Reactive) |
Pyridyldisulfide-Activated Beads (Cleavable, Thiol-Reactive) |
NHS-Activated Beads (Amine-Reactive) |
|---|---|---|---|
| Reactive Group | Iodoacetyl | 2-Pyridyl disulfide | NHS ester |
| Targets | Free thiol (-SH) groups on cysteine | Free thiol (-SH) groups on cysteine | Primary amine (-NH2) groups on lysine or N-terminus |
| Bond Formed | Thioether (non-cleavable) | Disulfide (cleavable) | Amide (non-cleavable) |
| Cleavable? | No | Yes (DTT or beta-mercaptoethanol) | No |
| pH Range | 7.2 – 9.0 | 4 – 8 (optimal: 4–5) | 7.0 – 9.0 |
| Reaction Time | ~2 hours | 15 min – 4 hours | 15–30 min (RT), 4 hrs at 4°C |
| Recommended For | Permanent immobilization of thiol-containing proteins/peptides | Reversible conjugation when recovery is needed | Fast, stable conjugation of amine-containing proteins/peptides |
| Steric Hindrance Solution | Long-arm version available | Long-arm version available | Long-arm version available |
| Applications | Affinity purification, immunoprecipitation, targeted protein capture | Reversible immobilization, pull-down assays, complex recovery | Affinity purification, antibody/peptide/DNA coupling, cell sorting |
| Binding Capacity | 15–20 µg ligand/mg beads | 15–20 µg ligand/mg beads | 15–20 µg ligand/mg beads |
| Advantages | Stable, irreversible bonding | Gentle ligand recovery possible | Fast reaction, high yield, no solvent waste |
LifeTein offers a wide variety of magnetic products. LifeTein’s unique coupling chemistry enables fast and efficient covalent conjugation of amino (-NH2) and thiol (-SH) containing proteins and peptides to the solid core with iron oxide clusters. All steps in the coupling are performed under physiological conditions at room temperature. The magnetic product consists of nano-superparamagnetic beads functionalized with high-density maleimide groups. These beads couple thiol-containing ligands like peptides, proteins, and antibodies, enabling magnetic separation of target proteins for downstream applications.
Magnetic Products:
| Cat. No | Product Name | Applications | Price | Order |
|---|---|---|---|---|
| LT16323 | Thiol-Activated Peptide Conjugation Magnetic Beads | Pull-down Assay, Protein Purification, Protein-Protein Interaction |  |
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| LTM007 | Iodoacetyl-Activated Peptide Conjugation Magnetic Beads | Pull-down Assay, Protein Purification, Protein-Protein Interaction | $350 | |
| LT13512 | Amine-activated Peptide Conjugation Magnetic Beads | Pull-down Assay, Protein Purification, Protein-Protein Interaction | $320 | |
| LTM003 | Streptavidin Magnetic Beads | Pull-down, Immunopurification | |
|
| LTM004 | Protein A Magnetic Beads | Antibody Purification | |
|
| LTM005 | Ni-NTA (His-tag Affinity) Magnetic Beads | Protein Purification, Protein-Protein Binding, Pull-down | |
|
| LTM006 | Glutathione Magnetic Beads | Protein Binding, Purification | |
