Glutathione Magnetic Beads

Magnetic bead peptide pulldown

Faster Magnetic Response; Simpler Protocols;

LifeTein provides a series of magnetic beads for any bioseparation application, from microliter scale to liter scale. Our Magnetic Beads are nano-superparamagnetic beads covalently coated with highly functional groups or compounds such as maleimide, primary amine, NHS (N-hydroxysuccinimide), carboxylic acid, purified streptavidin, protein A, reduced glutathione, nickel-charged nitrilotriacetic acid, and groups for DNA/RNA purification.

Magnetic bead
Name:
Glutathione Magnetic Beads
Catalog Number:
LTM006
Packing Details:
20mg in 20% Ethanol

The magnet is not provided.

Binding Capacity:
1-10 mg protein or 0.1-1mg peptide/mg magnetic beads.
Particle size:
500nm diameter
Coupling conditions:
Tris buffer , pH 7.2
Coupling capacity:
1-10 mg protein or 1 mg peptide/mg beads.

Coupling capacity was determined by incubating 30mg beads with human lgG (1 mg/ml in 1ml PBS) for 60 minutes at room temperature.

Storage:
Product shipped at room temp. Upon receipt, please store at 2-8°C.
Shelf Life:
Stable for at least two years
Description:

 

Glutathione Magnetic Beads are nano-superparamagnetic beads coupled with reduced glutathione. With a fast magnetic response rate, high protein binding capacity and low non-specific binding, the product provides an efficient and reliable method to purify GST (glutathione-S-transferase)-tagged fusion proteins from crude cell lysates.

 

Product Features:

Magnetic Separation – Fast response, complete purification process within 30 min, no chromatograph, no centrifugation, maximally preserve protein activity; Excellent Suspension Ability – Nanometer scale, rapid conversion from solid-phase to solution and vice versa; High Binding Capacity – efficient coupling, higher binding surface area; Low non-specific Binding –hydrophilic microspheres, low non-specific binding; Fully compatible with automation and LC-MS/MS analyses.

Specifications:

 

Diameter?500nm; pH stability?pH 3-13; 30 min sedimentation rate?<0.1%; Magnetic response rate: >30emu/g; Solvent?20% ethanol; Binding capacity: 10-100µg GST-tagged fusion protein per mg magnetic beads.


The product is not thoroughly tested and is not intended for human use. For in-vitro and research use only.
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