Protein A Magnetic Beads

Magnetic bead peptide pulldown

Faster Magnetic Response; Simpler Protocols;

LifeTein provides a series of magnetic beads for any bioseparation application, from microliter scale to liter scale. Our Magnetic Beads are nano-superparamagnetic beads covalently coated with highly functional groups or compounds such as maleimide, primary amine, NHS (N-hydroxysuccinimide), carboxylic acid, purified streptavidin, protein A, reduced glutathione, nickel-charged nitrilotriacetic acid, and groups for DNA/RNA purification.

Magnetic bead
Name:
Protein A Magnetic Beads
Catalog Number:
LTM004
Packing Details:
20mg in 10 mM Tris, 0.15 M NaCl, 0.1% BSA,1 mM EDTA, pH7.4, 0.1% NaN3

The magnet is not provided.

Binding Capacity:
1-10 mg IgG per ml of beads.
Particle size:
~ 1 um diameter
Coupling conditions:

Protein A Binding/Washing Buffer (57.7 mM Na2HPO4,42.3 mM NaH2PO4, pH 7.0)

Protein A Elution Buffer (0.2 M Glycine/HCl, pH 2.5)

Protein A Neutralization Buffer (1.0 M Tris-HCl, pH 9.0)

Coupling capacity:
1-10 mg protein or 1 mg peptide/mg beads.

Coupling capacity was determined by incubating 30mg beads with human lgG (1 mg/ml in 1 ml PBS) for 60 minutes at room temperature.

Storage:
Product shipped at room temp. Upon receipt, please store at 2-8°C.
Shelf Life:
Stable for at least two years
Description:

 

LifeTein Protein A Magnetic Beads are uniform, monodisperse, silica-based superparamagnetic beads covalently conjugated with ultrapure (Purity>97%) recombinant protein A on the surface. The Beads are specifically designed, tested, and quality-controlled for use in immunoprecipitation, and cell sorting when a selected primary antibody is used. The beads are also widely used for quick and efficient one-step purification of IgG antibodies from Serum samples, ascites fluid, plasma, or tissue culture supernatant from several species.

The binding affinity and specificity are summarized below.

Protein A Magnetic bead

 

Product Features:

Fast magnetic response,
High binding affinity,
Low non-specific binding,
Compatible with subsequent LC-MS/MS analyses,
Suitable for manual operation or full automation for high throughput analysis

Product Procedures:

 

The beads are simply added to a sample and antibodies will bind to the beads. After washing unbound impurities off, the antibodies can be eluted from the magnetic beads or the bound magnetic beads can be directly used in downstream experiments. The process can be completed manually or fully automated for high-throughput applications.

Download the Protocol HERE.


The product is not thoroughly tested and is not intended for human use. For in-vitro and research use only.
  • 1 Units in Stock

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LifeTein LifeTein provides custom peptide synthesis service, recombinant proteins, peptides, cytokines, custom antibody service and custom protein service. LifeTein is the world leader in fast peptide synthesis service with lab facility located in New Jersey USA. LifeTein Logo 100 Randolph Road, Suite 2D, Somerset USA New Jersey 08873
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