6His-Cysteine, HHHHHH-Cysteine

Product Name	6His-Cysteine, HHHHHH-Cysteine
Product Quantity	1mg
Catalog Number	LT12006
Molecular Weight	944.01
Formula	C39H49N19O8S
Sequence	His - His - His - His - His - His - Cys
Product Description	The HHHHHH-Cys peptide (6His) is used as a fusion tag for immunoaffinity chromatography, which is consisted of 6 amino acids (His-His-His-His-His-His-Cys) including a cysteine residue. The polyhistidine tags, or 6xHis-tags, are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Scientific Background	6His-Cysteine, HHHHHH-Cysteine is a synthetic hexahistidine (His6) tag-containing peptide. This product contains a hexahistidine sequence (HHHHHH). Polyhistidine tags coordinate immobilized transition-metal ions and are widely used in immobilized metal affinity chromatography, especially Ni-NTA and cobalt-based capture.
Research Applications	Ni-NTA or cobalt affinity-capture studies IMAC method development metal-binding comparisons His-tag accessibility controls
Experimental Notes	The behavior of a motif embedded in a longer peptide can differ from the isolated tag or parent sequence. Flanking residues, linkers, phosphorylation, fluorophores, affinity handles, cyclization, and terminal modifications may alter accessibility, binding, uptake, or assay performance.
Selected References	One-step purification of recombinant proteins with the 6xHis/Ni-NTA system Rapid evaluation of nickel binding properties of polyhistidine tags