Anti GST Monoclonal Antibody

Calatog Number:	LT0423
Lot Number:	411005100205
Isotype:	Mouse IgG2a
Clonality:	GST.B6
Quantity:	50 μg
Immunogen:	GST protein
Specificity:	Recognizes native and denatured forms of purified GST or GST fusion proteins
Purification:	Protein A affinity chromatography from mouse ascites fluid
Applications:	Dot, ELISA, IS, IP, WB
Sensitivity:	WB (with ECL): 10-50ng of purified GST or GST fusion proteins
Working Condition:	WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:	Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:	Fig. 1:5000 (0.2µg/mL) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein