Anti HA Monoclonal Antibody

Catalog Number:
LT0422
Lot Number:
411005100206
Isotype:
Mouse IgG3
Clonality:
HA.C5
Quantity:
50 μg, lyophilized with PBS, pH7.4
Positive Control:
Purchase matching Western Blot positive control protein
Immunogen:
YPYDVPDYA (HA) synthetic peptide conjugated to KLH
Specificity:
Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot. ELISA, IP, IS, WB
Sensitivity:
WB (with ECL): 1-5ng of HA-tagged fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-HA mAb dilution probed against HEK293 cells transfected with HA-tagged protein vector
Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)

Citations:

Xyloglucan xylosyltransferases XXT1, XXT2, XXT5, and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes

YH Chou, G Pogorelko - Plant Physiology, 2012

...membrane (0.2 μm, Bio-Rad) for immunodetection. Monoclonal anti-GFP antibodies (MMS- 118P,Covance) were used (1:5000 dilution) for detection of YFP. Monoclonal anti-HA antibodies (LT0422, LifeTein) were used (1:500 dilution) to detect HA-fused proteins. Page 21. 21 ...

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