Anti-RFP Monoclonal Antibody

Catalog Number:
LT9993
Lot Number:
411005100209
Isotype:
Mouse IgG1
Clonality:
RF5R
Quantity:
50 μg, lyophilized with PBS, pH7.4
Immunogen:
RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates
Specificity:
Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, tdTomato, etc
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot, ELISA, IP, IS, WB
Sensitivity:
WB (with ECL): 1-10ng of purified RFP or RFP fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-RFP mAb dilution probed against HEK293 cells transfected with RFP vector
Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with RFP vector; untransfected control (1), transfected with TurboRFP (2), and transfected with dsRed (3)
  • 45 Units in Stock

$110.00

Qty Discounts Off Price
1-9
$110.00
10-19
$99.00
20+
$88.00
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