| LT9999 |
| 411005100207 |
| Mouse IgG2b |
| BA3R |
| 50 μg, lyophilized with PBS, pH7.4 |
| β-Actin N-terminal peptide-KLH conjugates |
| Recognizes native and denatured forms of β-Actin (~42kDa) |
| β-Actin from human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectable |
| Protein A affinity chromatography from mouse ascites fluid |
| Dot, ELISA, IS, WB |
| WB (with ECL): 10-50ng of purified GST or GST fusion proteins |
| WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
| 
Fig. 1:1000 (1µg/mL) Ab dilution used in WB of 20µg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4) |