Anti-β-Actin Monoclonal Antibody

Catalog Number:
LT9999
Lot Number:
411005100207
Isotype:
Mouse IgG2b
Clonality:
BA3R
Quantity:
50 μg, lyophilized with PBS, pH7.4
Immunogen:
β-Actin N-terminal peptide-KLH conjugates
Specificity:
Recognizes native and denatured forms of β-Actin (~42kDa)
Cross Reactivity:
β-Actin from human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectable
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot, ELISA, IS, WB
Sensitivity:
WB (with ECL): 10-50ng of purified GST or GST fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-β-Actin mAb dilution used in WB of 20µg/lane tissue lysates from human
Fig. 1:1000 (1µg/mL) Ab dilution used in WB of 20µg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)
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