Anti-GAPDH Monoclonal Antibody

Catalog Number:
LT9995
Lot Number:
411005100211
Isotype:
Mouse IgG1
Clonality:
GA1R
Quantity:
50 μg, lyophilized with PBS, pH7.4
Immunogen:
Recombinant GAPDH
Specificity:
Recognizes native and denatured forms of GAPDH (~37kDa)
Cross Reactivity:
GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot, ELISA, IS, WB
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-gadph mAb dilution used in WB of lane tissue lysates from human etc.anti-gadph mAb dilution used in WB of lane tissue lysates from human etc.
Fig. LEFT: 1:2000 (0.5µg/mL) Ab dilution used in WB of 5µg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)
  • 48 Units in Stock

$210.00  $110.00
Save: 48% off

Qty Discounts Off Price
1-9
$110.00
10-19
$99.00
20-29
$93.50
30+
$88.00
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