Anti His Monoclonal Antibody

Catalog Number:
Lot Number:
Mouse IgG2b
50 μg, Lyophilized with PBS, pH7.4
Positive Control:
Purchase matching Western Blot positive control protein
HHHHHH (6x His) synthetic peptide conjugated to KLH
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Protein A affinity chromatography from mouse ascites fluid
WB (with ECL): 1-2ng of His-tagged fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
anti-His mAb on His-tagged fusion proteins in HEK293 cells
Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins
Fig. 02 Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5µg/mL)
Standard ladder containing five different His-tagged proteins
Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)

1. Membrane association of monotopic phosphoglycosyl transferase underpins function, Nature Chemical Biology, volume 14, pages538–541 (2018), doi:10.1038/s41589-018-0054-z.

...The primary antibody, mouse anti-His (LifeTein, cat. no. LT0426) was applied in a 1:3,000 dilution for 1?h...

2. Cross-inhibition of ChikunGunya virus fusion and infection by alphavirus E1 domain III proteins, Claudia Sa¡nchez San Martin, Soumya Nanda, Yan Zheng, Whitney Fields and Margaret Kielian, Journal of Virology, Published ahead of print 1 May 2013, doi: 10.1128/JVI.00814-13

... and a mAb against the His Tag (Lifetein LLC, South Plainfield, NJ) to detect His-tagged CHIKV...

3. 14-3-3 Proteins Restrain the Exo1 Nuclease to Prevent Over-resection, The Journal of Biological Chemistry, First Published on April 1, 2015, doi: 10.1074/jbc.M115.644005 jbc.M115.644005.

... ... Anti-FLAG, anti-His (LifeTein, LT0426), anti-14-3-3 (Cell Signaling, 8312), antibodies were purchased from the...

Anti His-tagged proteins
  • 9 Units in Stock

Ask a Question

$228.00 $80.00Save: 65% off

Add to Cart:

For more information, please visit this product's webpage.

Customers who bought this product also purchased...

$300.00 $249.00Save: 17% off
$264.00 $150.00Save: 43% off
$240.00 $190.00Save: 21% off
$264.00 $150.00Save: 43% off
$300.00 $149.00Save: 50% off
LifeTein LifeTein provides custom peptide synthesis service, recombinant proteins, peptides, cytokines, custom antibody service and custom protein service. LifeTein is the world leader in fast peptide synthesis service with lab facility located in New Jersey USA. LifeTein Peptide 100 Randolph Road, Suite 2D, Somerset USA New Jersey 08873
Copyright © 2024 Powered by LifeTein