Anti-V5 Monoclonal Antibody Anti V5 Monoclonal Antibody [LT0428] - $50.00 : LifeTein, The peptide synthesis service company [LT9992] - $150.00 : LifeTein, The Peptide Synthesis Service Company

Anti-V5 Monoclonal Antibody

Catalog Number:	LT9992
Lot Number:	411005100208
Isotype:	Mouse IgG1
Clonality:	E10
Quantity:	50 μg, lyophilized with PBS, pH7.4
Immunogen:	GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Specificity:	Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins
Purification:	Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Applications:	Dot, ELISA, IP, IS, WB
Sensitivity:	WB (with ECL): 1-2ng of V5-tagged fusion proteins
Working Condition:	WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:	Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:	Fig. 1:000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)