Anti-V5 Monoclonal Antibody

Catalog Number:
LT9992
Lot Number:
411005100208
Isotype:
Mouse IgG1
Clonality:
E10
Quantity:
50 μg, lyophilized with PBS, pH7.4
Immunogen:
GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Specificity:
Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins
Purification:
Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Applications:
Dot, ELISA, IP, IS, WB
Sensitivity:
WB (with ECL): 1-2ng of V5-tagged fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-V5 mAb dilution probed against HEK293 cells transfected with V5-tagged protein vector
Fig. 1:000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)
  • 45 Units in Stock

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