| LT9992 |
| 411005100208 |
| Mouse IgG1 |
| E10 |
| 50 μg, lyophilized with PBS, pH7.4 |
| GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH |
| Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins |
| Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor |
| Dot, ELISA, IP, IS, WB |
| WB (with ECL): 1-2ng of V5-tagged fusion proteins |
| WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
| 
Fig. 1:000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2) |