Anti-GFP Monoclonal Antibody

Catalog Number:
LT9994
Lot Number:
411005100210
Isotype:
Mouse IgG1
Clonality:
GF28R
Quantity:
50 μg, Lyophilized with PBS, pH7.4
Positive Control:
Purchase matching Western Blot positive control protein
Immunogen:
GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Specificity:
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot, ELISA, IP, IS, WB
Sensitivity:
WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-GFP mAb dilution probed against HEK293 cells transfected with GFP-tagged protein vector
Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1µg (2) and 10µg (3) of cell lysates used
  • 21 Units in Stock

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