| LT9991 |
| 411005100208 |
| Mouse IgG2a |
| BT7R |
| 50 μg, lyophilized with PBS, pH7.4 |
| β-Tubulin N-terminal peptide-KLH conjugates |
| Recognizes native and denatured forms of β-Tubulin (~50kDa) |
| β-ATubulin from human, monkey, mouse, rat, rabbit, chicken. β-Tubulin from other species may also be detectable |
| Protein A affinity chromatography from mouse ascites fluid |
| Dot, ELISA, IS, WB |
| WB (with ECL): 1:2000-5000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test |
| Store at -20°C for 2 years or more. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C (add 0.05% NaN3) for several days to weeks. |
| 
Fig.01 LifeTein™s three loading control mAbs reacting against 10μg/lane of mouse brain tissue lysates. 50kD band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5μg/mL); 42kD band is Anti-β-Actin (BA3R) at 1:1000 dilution (1μg/mL); 37kD band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2μg/mL)
Fig.021:4000 (0.25μg/mL) Ab dilution used in WB of 10μg/lane mouse brain tissue lysates |