Anti β-Tubulin Monoclonal Antibody

Catalog Number:
LT9991
Lot Number:
411005100208
Isotype:
Mouse IgG2a
Clonality:
BT7R
Quantity:
50 μg, lyophilized with PBS, pH7.4
Immunogen:
β-Tubulin N-terminal peptide-KLH conjugates
Specificity:
Recognizes native and denatured forms of β-Tubulin (~50kDa)
Cross Reactivity:
β-ATubulin from human, monkey, mouse, rat, rabbit, chicken. β-Tubulin from other species may also be detectable
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot, ELISA, IS, WB
Working Condition:
WB (with ECL): 1:2000-5000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for 2 years or more. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C (add 0.05% NaN3) for several days to weeks.
Images:
 LifeTein™s three loading control mAbs reacting against 10μg/lane of mouse brain tissue lysates
Fig.01 LifeTein™s three loading control mAbs reacting against 10μg/lane of mouse brain tissue lysates. 50kD band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5μg/mL); 42kD band is Anti-β-Actin (BA3R) at 1:1000 dilution (1μg/mL); 37kD band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2μg/mL)
1:4000 (0.25μg/mL) anti-β-Tubulin mAb dilution used in WB of 10μg/lane mouse brain tissue lysates
Fig.021:4000 (0.25μg/mL) Ab dilution used in WB of 10μg/lane mouse brain tissue lysates
  • 50 Units in Stock

$210.00  $150.00
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$127.50
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$120.00
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