HA tag peptide C-YPYDVPDYA

The peptide sequence YPYDVPDYA is widely recognized as the HA tag, a 9-amino-acid epitope derived from the human influenza hemagglutinin (HA) protein. This tag is extensively utilized in molecular biology to facilitate the detection, purification, and analysis of recombinant proteins.

Functional Overview

• Origin: The HA tag corresponds to amino acids 98–106 of the influenza virus HA protein.

• Sequence: YPYDVPDYA

• Applications:

  • Protein Detection: The HA tag is recognized by specific monoclonal antibodies, enabling the detection of tagged proteins through techniques such as Western blotting, immunofluorescence, and immunoprecipitation.

  • Protein Purification: HA-tagged proteins can be purified using affinity chromatography methods that exploit the high-affinity interaction between the tag and anti-HA antibodies.

  • Protein Localization and Interaction Studies: The tag facilitates studies on protein localization within cells and interactions with other proteins.

• Versatility: Due to its small size, the HA tag can be fused to either the N-terminus or C-terminus of target proteins without significantly altering their function or localization.

Research and Development

The HA tag has been instrumental in various research applications:

• Epitope Tagging: It serves as a model epitope tag in studies aiming to understand protein expression and function.

• Antibody Production: Synthetic peptides corresponding to the HA tag are used to generate monoclonal and polyclonal antibodies, which are critical tools in protein research.

• Biotinylation: Biotinylated versions of the HA tag are available, allowing for enhanced detection and purification using streptavidin-based systems.

The YPYDVPDYA sequence, known as the HA tag, is a valuable tool in molecular biology for the detection and purification of recombinant proteins. Its small size and the availability of high-affinity antibodies make it an ideal choice for various applications in protein research.

HA tag peptide C-YPYDVPDYA is a 9-residue synthetic peptide with the sequence Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala. The sequence contains 3 aromatic residues that can contribute to hydrophobic or aromatic interactions; has a relatively high hydrophobic-residue fraction that can influence aqueous solubility and surface adsorption. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence.

• LC-MS/HPLC analytical method development
• sequence-specific assay controls

Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions.