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The peptide sequence YPYDVPDYA is widely recognized as the HA tag, a 9-amino-acid epitope derived from the human influenza hemagglutinin (HA) protein. This tag is extensively utilized in molecular biology to facilitate the detection, purification, and analysis of recombinant proteins.
Functional Overview
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Origin: The HA tag corresponds to amino acids 98–106 of the influenza virus HA protein.
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Sequence: YPYDVPDYA
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Applications:
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Protein Detection: The HA tag is recognized by specific monoclonal antibodies, enabling the detection of tagged proteins through techniques such as Western blotting, immunofluorescence, and immunoprecipitation.
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Protein Purification: HA-tagged proteins can be purified using affinity chromatography methods that exploit the high-affinity interaction between the tag and anti-HA antibodies.
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Protein Localization and Interaction Studies: The tag facilitates studies on protein localization within cells and interactions with other proteins.
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Versatility: Due to its small size, the HA tag can be fused to either the N-terminus or C-terminus of target proteins without significantly altering their function or localization.
Research and Development
The HA tag has been instrumental in various research applications:
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Epitope Tagging: It serves as a model epitope tag in studies aiming to understand protein expression and function.
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Antibody Production: Synthetic peptides corresponding to the HA tag are used to generate monoclonal and polyclonal antibodies, which are critical tools in protein research.
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Biotinylation: Biotinylated versions of the HA tag are available, allowing for enhanced detection and purification using streptavidin-based systems.
The YPYDVPDYA sequence, known as the HA tag, is a valuable tool in molecular biology for the detection and purification of recombinant proteins. Its small size and the availability of high-affinity antibodies make it an ideal choice for various applications in protein research.
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