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The peptide sequence GNYTCEVTELTREGETIIELK is a synthetic "self-peptide" derived from the extracellular domain of human CD47, a transmembrane protein that interacts with signal regulatory protein alpha (SIRPα) on macrophages and other phagocytic cells. This interaction delivers a well-characterized "don't eat me" signal, which suppresses phagocytosis and enables immune evasion.
The FITC-Ahx-GNYTCEVTELTREGETIIELK peptide is a fluorescently labeled version designed for cellular imaging, binding assays, and nanoparticle tracking studies. The Ahx (aminohexanoic acid) linker provides spatial separation between the fluorophore and peptide, preserving biological activity while enabling accurate fluorescence detection.
Mechanism of Action: CD47–SIRPα Immune Checkpoint
CD47 is widely expressed on healthy cells and is frequently overexpressed in cancer cells to evade immune surveillance. The GNYTCEVTELTREGETIIELK peptide corresponds to a key binding motif responsible for interaction with SIRPα receptors. By mimicking this interface, the peptide can engage SIRPα and trigger inhibitory signaling pathways that suppress macrophage-mediated phagocytosis.
This mechanism has been extensively explored in nanomedicine and drug delivery systems, where surface functionalization with CD47-derived peptides reduces clearance by the mononuclear phagocyte system (MPS).
Applications in Nanoparticle Drug Delivery
- Nanoparticle surface functionalization to reduce macrophage uptake
- Prolonged systemic circulation of drug delivery systems
- Enhanced tumor accumulation via immune evasion strategies
- Improved delivery efficiency of liposomes, polymeric nanoparticles, and lipid nanoparticles (LNPs)
Fluorescently labeled versions such as FITC-Ahx-GNYTCEVTELTREGETIIELK are particularly useful for:
- Cell binding and receptor interaction studies
- Nanoparticle tracking and biodistribution analysis
- Flow cytometry and confocal microscopy experiments
Applications in Immunoengineering and Therapeutics
- Immune evasion strategies in drug delivery systems
- Cancer immunotherapy research (CD47–SIRPα axis)
- Biomaterial surface engineering
- Anti-phagocytic coating development for biologics
Advantages of CD47-Derived Self Peptide
- Mimics natural "self-recognition" signaling pathway
- Reduces immune clearance of nanoparticles and therapeutics
- Compatible with lipid nanoparticles, polymers, and proteins
- Available in multiple formats for imaging, conjugation, and delivery
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