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FITC-Ahx-CD47 peptide is a fluorescent CD47-mimetic probe designed for visualization of peptide binding, peptide distribution, and CD47-related interaction studies. This product combines a CD47-derived recognition sequence with a fluorescein (FITC) reporter and a flexible aminohexanoic acid spacer, allowing researchers to track the peptide in fluorescence-based assays while reducing steric interference between the fluorophore and the peptide binding region.
CD47 is widely recognized as a “don’t eat me” signal that engages signal regulatory protein alpha (SIRPα) on macrophages and other myeloid cells. CD47-derived peptides have been used to study this checkpoint pathway and to reduce macrophage uptake of engineered nanocarriers. In published nanoparticle studies, CD47-peptide surface modification decreased RAW264.7 macrophage internalization and supported direct experimental evaluation of CD47-peptide / SIRPα interaction.
Why Use FITC-Ahx-CD47 Peptide?
Compared with an unlabeled CD47 peptide, the FITC-Ahx format is particularly useful when the experimental goal is not only receptor engagement but also direct optical readout. The Ahx linker helps spatially separate the FITC group from the peptide sequence, making this format suitable for fluorescence microscopy, flow cytometry, uptake experiments, and probe-development workflows.
Key Research Uses
- Fluorescence-based peptide binding studies
- Cell-surface interaction analysis involving CD47 / SIRPα
- Flow cytometry evaluation of peptide-associated cell binding
- Confocal or fluorescence microscopy localization experiments
- Nanoparticle and carrier-surface peptide tracking studies
- Competitive binding and receptor-blocking assay development
Applications in Drug Delivery and Immunology Research
Macrophage Interaction Studies
FITC-Ahx-CD47 peptide can be used to investigate how CD47-mimetic ligands interact with phagocytic cells. This is valuable for studies examining immune evasion, macrophage recognition, and modulation of particle uptake.
Nanomedicine and Surface Engineering
CD47-derived peptides have been reported in nanocarrier systems designed to reduce phagocytic clearance. A fluorescently labeled version is useful for monitoring peptide presentation on delivery systems and for correlating peptide display with particle uptake behavior.
Immune Checkpoint Research
The CD47–SIRPα axis is an important innate immune checkpoint in oncology and biomaterials research. FITC-Ahx-CD47 peptide provides a convenient fluorescent tool for screening, assay development, and mechanistic studies focused on this pathway.
Probe and Assay Development
Because this peptide contains both a targeting sequence and a fluorophore, it is suitable for building cell-based assays, competitive interaction assays, and fluorescence readouts for peptide optimization programs.
Advantages of the FITC-Ahx Format
- Direct fluorescence detection without secondary labeling
- Useful for microscopy and flow cytometry workflows
- Ahx spacer improves flexibility between FITC and peptide motif
- Suitable for localization, tracking, and binding studies
- Convenient companion product to unlabeled CD47 peptide formats
Recommended Companion Products
For researchers who need functional comparison between labeled and unlabeled formats, we recommend running this product alongside our
plain CD47 peptide.
If your study focuses on the self-recognition motif in nanoparticle or surface-coating applications, see our
self peptide.
Research Use Only. Not for human or therapeutic use.
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