| Biotin, Ahx linker, amidation; The majority of Staphylococcus aureus virulence- and colonization-associated surface proteins contain a pentapeptide recognition motif (LPXTG). This motif can be recognized and cleaved by sortase A (SrtA) which is a membrane-bound transpeptidase. LPTEG-tagged probes and proteins can be conjugated using sortase A in a single step to glycines naturally exposed at the cell surface. |