Catalog Number | LT8832 |
Product Name | Thrombin Fluorogenic Substrate, H-D-Phe-Pro-Arg-AFC |
Product Quantity | 10 mg |
Sequence | H-D-Phe-Pro-Arg-AFC |
Molecular Weight | 629.6 |
Formula | C30H34F3N7O5 |
Purity | ≥95% |
Scientific Background | H-D-Phe-Pro-Arg-AFC is a fluorogenic tripeptide substrate for thrombin and related arginine-cleaving serine proteases. Cleavage releases AFC and converts protease activity into a fluorescence signal. D-Phe-Pro-Arg-containing substrates are established tools for thrombin assay development and coagulation-protease measurements. |
Research Applications | - thrombin activity assays
- coagulation protease studies
- continuous fluorogenic enzyme kinetics
- thrombin inhibitor screening
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Experimental Notes | AFC fluorescence is typically measured near 380 nm excitation and 500 nm emission. Short Arg-terminated substrates may also be processed by related serine proteases, so thrombin specificity should be established experimentally. |
Selected References | - New Fluorogenic Substrates for α-Thrombin, Factor Xa, Kallikreins, and Urokinase
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