Thrombin Fluorogenic Substrate, D-Phe-Pro-Arg-AFC

H-D-Phe-Pro-Arg-AFC is a fluorogenic peptide substrate for thrombin and related trypsin-like serine proteases. Thrombin cleavage after arginine liberates AFC, producing an increase in green fluorescence suitable for continuous enzyme-activity measurements. The D-Phe-Pro-Arg recognition motif is widely used in thrombin substrate and inhibitor studies.

• thrombin activity assays
• coagulation research
• serine-protease kinetics
• thrombin inhibitor screening

Other trypsin-like proteases may also cleave Arg-AFC substrates. Use purified thrombin or appropriate selective inhibitors when enzyme assignment is important. Protect AFC-containing substrates from light.

1. Amino acid and peptide derivatives of rhodamine as fluorogenic substrates for protease assays