Sortase A FRET Substrate II, Dabcyl-QALPETGEE-EDANS

Dabcyl-QALPETGEE-EDANS is an internally quenched peptide substrate for Gram-positive bacterial sortases. The central LPETG motif is a canonical Sortase A recognition sequence, while the flanking residues provide a longer sorting-signal-like context than the minimal LPETG substrate. Cleavage separates the Dabcyl quencher from EDANS and generates a fluorescence increase suitable for continuous enzyme assays.

• Sortase A activity assays
• sortase inhibitor screening
• LPXTG substrate-recognition studies
• bacterial transpeptidase research

The extended flanking sequence can influence substrate kinetics relative to shorter LPETG FRET constructs. Use the same substrate format when comparing inhibitor potency or enzyme variants.

1. Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif
2. Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope