Sortase A FRET Substrate III, Abz-LPETG-K(Dnp)-NH2

Abz-LPETG-K(Dnp)-NH2 is an internally quenched Sortase A substrate built around the LPETG recognition motif. The N-terminal anthraniloyl (Abz) fluorophore is quenched by the Dnp group before cleavage. Sortase-mediated processing of the LPETG sequence separates reporter and quencher, allowing sensitive fluorescence-based measurements of enzyme activity and substrate specificity.

• Sortase A activity assays
• sortase substrate-specificity studies
• sortase inhibitor screening
• FRET transpeptidase assays

The Abz/Dnp reporter pair gives this construct different optical and kinetic properties from Dabcyl/EDANS sortase substrates. Use matched substrate chemistry for quantitative comparisons.

1. Engineering the Substrate Specificity of Staphylococcus aureus Sortase A