Catalog Number | LT8796 |
Product Name | Sortase A FRET Substrate I, DABCYL-LPETG-EDANS |
Product Quantity | 1 mg |
Sequence | DABCYL-LPETG-EDANS |
Molecular Weight | 1015.2 |
Purity | ≥95% |
Scientific Background | DABCYL-LPETG-EDANS is an internally quenched FRET substrate containing the canonical LPXTG recognition motif of Staphylococcus aureus Sortase A. Sortase A cleaves between threonine and glycine in LPXTG-containing sorting signals and forms a transpeptidation intermediate used to anchor surface proteins to the Gram-positive cell wall. Separation of the DABCYL quencher and EDANS fluorophore upon cleavage enables continuous fluorescence-based monitoring of sortase activity. |
Research Applications | - Sortase A activity assays
- sortase inhibitor screening
- LPXTG recognition studies
- transpeptidase assay development
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Experimental Notes | Fluorescence can be monitored using EDANS-compatible excitation/emission settings. The substrate reports cleavage of the LPETG motif but does not reproduce every structural feature of a full-length cell-wall sorting signal. |
Selected References | - Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif
- Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
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