Recombinant Human ACE2 Protein(H374N, and H378N)

Featured Product New!: Monoclonal Antibodies to Coronavirus SARS-CoV-2 RBD Domain

Description: Recombinant Human ACE2 Protein(H374N, and H378N)

Product: Recombinant protein of the extracellular domain (Gln18-Asp615) of Homo sapiens angiotensin converting enzyme 2 (ACE2), transcript variant 2 (NM_021804), with H374N, H378N point mutation, and a Human IgG1 Fc tag.

Cat #: LTP-V005

Host: Human cells

RefSeq: NM_021804; NP_068576; UniProt#: Q9BYF1; GeneID: 59272;

Molecular Weight: 97.8 kDa

Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only!

Background: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008].

Quantity: 50ug, >Endotoxin level is < 0.1 ng/µg of protein (<1EU/µg)

Purity: >90% by SDS-PAGE gel and Coomassie Blue staining

Formulation: Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives

Download Data File:download pdf data sheet

Recombinant Human ACE2 Protein

Goal: To assess the binding of anti-SARS CoV-2 RBD mAb, hACE2 and SARS CoV-19-infected Patient Sera to S1 and RBD Proteins

Methods: Oriented Capture and Covalent Immobilization of Ligands

  • SARS CoV-2 S1 Spike Protein – Polyhistidine Tagged (LifeTein Product LTP-V006);
  • SARS CoV-2 RBD Protein – Polyhistidine Tagged (LifeTein Product LTP-V009);
  • Recombinant hACE2, human IgG1 Fc-Tagged (LifeTein Product LTP-V003)
  • Anti-SARS CoV-2 Spike RBD mAb: Active Motif (Ab-LTA003) from COVID-19 patients who have cleared the virus. Patient serum IgG sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells
  • Patient serum samples from COVID-19
  • Normal Human Pooled Serum (NHPS)

Coronavirus-spike-protein.png

Binding of hACE2 to S1 and RBD Proteins. The low binding of S1 and hACE2 could be caused by the closed SARS-CoV-2 S1 trimer.

Reference: Walls et al., 2020, Cell 180, 281–292, April 16, 2020, https://www.cell.com/cell/pdf/S0092-8674(20)30262-2.pdf

Coronavirus-spike-protein.png

Binding of anti-SARS CoV-2 mAb (Ab-LTA003) to S1 and RBD Proteins

Summary:

  • hACE2 binds strongly to RBD and slightly to S1
  • mAb (Ab-LTA003) binds strongly to RBD but not to S1
  • Normal Human Pooled Serum does not bind to RBD or S1 (Data not shown)
  • Human Sera from SARS CoV-19 infected patients (PCR-confirmed) bind to RBD and minimally to S1 (Data not shown)
  • The low binding of S1 and hACE2 could be caused by the closed SARS-CoV-2 S1 trimer

Coronavirus spike protein with ACE2

Coronavirus spike proteins bind to ACE2

The S1 RBD binds to the ACE2 enzyme, using the Fc only as a negative control, which the RBD did not bind to. The assay was performed with an ELISA, detecting the RBD with streptavidin HRP.

  • 5 Units in Stock

Ask a Question

$960.00

Add to Cart:
LifeTein LifeTein provides custom peptide synthesis service, recombinant proteins, peptides, cytokines, custom antibody service and custom protein service. LifeTein is the world leader in fast peptide synthesis service with lab facility located in New Jersey USA. LifeTein Peptide 100 Randolph Road, Suite 2D, Somerset USA New Jersey 08873
Copyright © 2024 LifeTein.com. Powered by LifeTein