Monoclonal Antibodies to Coronavirus SARS-CoV-2 RBD Domain

Coronavirus antibody

Catalog Number: LTA-V010

Name: SARS-CoV-2 Spike RBD Monoclonal Antibody

Immunogens: Utilizes 2019 novel Coronavirus spike protein receptor binding domain as the immunogens for monoclonal antibody production

Antibody Subtype: IgG1

Concentration: 200ug, 0.4mg/ml, Lyophilized in Phosphate Buffered Saline, pH7.4, (10mg in stock, Call for Price)

Advantages: Chemically synthesized peptides were oxidized to form the correct disulfide bridge. The peptides were folded in a proprietary folding solution to maintain the native protein structure

Progress Update: ELISA to be tested, Hybridoma cell fusion to be performed, Heavy/Light chains to be sequenced, Genes to be constructed, Proteins to be expressed in a mammalian cell lines, Antibodies to be tested and characterized.

ELISA Results: Coating Antigens: SARS-CoV-2 Spike RBD; Coating Concentration: 2 ug/ml, 100ul / well; Coating Buffer: Phosphate Buffered Saline, pH7.4; Secondary Antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated;

Coronavirus antibody ELISA

Western Blot: Western Blots against SARS-CoV-2 RBD Protein, 6His tagged.

Coronavirus antibody Western

Antibody Binding to Trimers: Antibodies were captured to the surface of SARS-CoV-2 S Trimer Proteins

Coronavirus antibody Binds to Trimer

Antibody Binding to RBD Protein: Antibodies can bind to immobilized SARS-CoV-2 RBD 6His tagged protein tightly.

Coronavirus antibody binds to RBD

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