Plasmin Fluorogenic Substrate, D-Val-Leu-Lys-AFC

H-D-Val-Leu-Lys-AFC is a short fluorogenic serine-protease substrate used to monitor plasmin activity. Proteolytic cleavage at the Lys-AFC bond releases the AFC fluorophore, allowing continuous fluorescence measurements near 380 nm excitation and 500 nm emission. The D-Val residue contributes resistance to nonspecific aminopeptidase processing and helps define the substrate's protease-recognition profile.

• plasmin activity assays
• fibrinolysis research
• serine-protease kinetics
• protease inhibitor screening

Fluorescence intensity depends on AFC release, instrument settings and buffer composition. Plasmin selectivity should be confirmed in mixed protease samples with enzyme-specific inhibitors or orthogonal controls.

1. New Fluorogenic Peptide Substrates for Plasmin