PKC-epsilon Peptide Substrate, ERMRPRKRQGSVRRRV

ERMRPRKRQGSVRRRV is a basic peptide substrate developed from the pseudosubstrate region of protein kinase C epsilon (PKCε), with a serine introduced at the phosphoacceptor position. Early biochemical studies of PKCε demonstrated distinct substrate-recognition properties relative to other PKC isozymes, and this sequence provides a defined reagent for measuring PKCε-dependent phosphorylation and comparing PKC isozyme activity.

• PKC-epsilon kinase activity assays
• PKC isozyme substrate studies
• phosphorylation kinetics
• PKC inhibitor screening

The N-terminal glutamate can undergo pyroglutamate formation under some conditions. For quantitative kinase work, standardize storage, reconstitution, and assay timing.

1. Unique substrate specificity and regulatory properties of PKC-epsilon: a rationale for diversity
2. Expression, purification, and characterization of protein kinase C-epsilon