| Product Name | Histone H4 (1-25) K5/K8/K12/K16 Tetra-Acetyl-GSGSK(Biotin) |
| Product Quantity | 1 mg |
| Sequence | SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVLRDN-GSGSK(Biotin) |
| Molecular Weight | 3401 |
| Purity | ≥95% |
| Mechanism & Biological Significance | This hyperacetylated H4 tail carries acetylation at all four canonical H4 N-terminal lysines (K5, K8, K12 and K16). The construct models a strongly acetylated chromatin state and is useful as a multivalent acetyl-lysine ligand for reader-protein and antibody specificity studies. |
| Published Research Context | A peer-reviewed histone-variant study reports the exact H4K5/K8/K12/K16-acetylated sequence SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVLRDNGSGS-K(Biotin) in its dot-blot peptide panel. This directly supports the same H4(1-25)-GSGSK(Biotin) chemistry rather than a shorter H4(1-21) or GGK(Biotin) substitute. |
| Research Applications | - acetyl-histone antibody specificity controls
- bromodomain/acetyl-lysine reader assays
- histone deacetylation comparisons
- dot-blot and streptavidin-based assays
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| Experimental Considerations | The current vendor page reports MW 3401 and price $522. The older Batch 67 source lists MW 3443.1, a 42-Da discrepancy consistent with one extra acetyl group; the vendor-supported value is used here and the discrepancy is flagged. |
| Selected Scientific References | - The P. falciparum alternative histones PfH2A.Z and PfH2B.Z are dynamically acetylated
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