| Product Name | Histone H4 (1-21)-GGK(Biotin) |
| Product Quantity | 1 mg |
| Sequence | Ac-SGRGKGGKGLGKGGAKRHRKV-GGK(Biotin) |
| Molecular Weight | 2602.2 |
| Purity | ≥95% |
| Mechanism & Biological Significance | This N-terminally acetylated H4(1-21) peptide carries a C-terminal GGK(Biotin) capture handle. The H4 tail contains Arg3 as well as major lysine acetylation sites and can serve as an immobilizable substrate for histone-modifying enzymes and interaction assays. |
| Published Research Context | The exact biotinylated H4 sequence ac-SGRGKGGKGLGKGGAKRHRKVGGK(Biotin) has been used as the substrate in radioactive PRMT1 methylation assays for small-molecule inhibitor profiling. This establishes direct assay use of the same H4 tail, N-terminal acetylation and C-terminal biotin capture chemistry. |
| Research Applications | - PRMT1 methylation assays
- histone methyltransferase inhibitor screening
- streptavidin capture workflows
- chromatin-enzyme assay development
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| Experimental Considerations | The N-terminal acetyl group and C-terminal biotinylated lysine are integral to this reagent. Results should not be assumed to match free H4(1-21) or differently linked biotin constructs. |
| Selected Scientific References | - Design and Synthesis of Novel PRMT1 Inhibitors and Investigation of Their Effects on the Migration of Cancer Cell
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