Histone H4 (1-21), p300/CBP Substrate

Histone H4(1-21) is the unmodified N-terminal H4 tail and contains the major acetylation sites Lys5, Lys8, Lys12 and Lys16. The fragment contains determinants required for recognition by p300/CBP histone acetyltransferases and is useful as a defined substrate for histone-acetylation studies.

• p300/CBP histone acetyltransferase assays
• H4 acetylation studies
• chromatin enzyme kinetics
• epigenetic inhibitor screening

This is the unmodified free-acid H4(1-21) sequence. It is chemically distinct from N-terminally acetylated and biotinylated H4 constructs and serves as a useful baseline substrate.

1. Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro