| Product Name | Histone H4 (1-21) K5/K8/K12/K16 Tetra-Acetyl-GGK(Biotin) |
| Product Quantity | 1 mg |
| Sequence | SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKV-GGK(Biotin) |
| Molecular Weight | 2728.3 |
| Purity | ≥95% |
| Mechanism & Biological Significance | This H4 tail contains acetylation at Lys5, Lys8, Lys12 and Lys16, creating a hyperacetylated H4 ligand with markedly reduced positive charge and multiple acetyl-lysine recognition sites. The terminal biotin enables immobilization in displacement, pull-down and reader-protein assays. |
| Published Research Context | The exact tetra-acetyl H4(1-21)-GGK(Biotin) sequence has been used directly in AlphaScreen peptide-displacement assays for BRD4 and in biochemical screening of SMARCA2 bromodomain inhibitors. These studies demonstrate that the construct functions as a multivalent acetyl-histone reader ligand in drug-discovery assay formats. |
| Research Applications | - BRD4 bromodomain inhibitor screening
- SMARCA2 bromodomain assays
- acetyl-histone reader profiling
- AlphaScreen and streptavidin-based displacement assays
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| Experimental Considerations | Combinatorial tetra-acetylation is not equivalent to any single H4 acetylation state. Use matched unmodified or partially acetylated H4 controls when interpreting reader selectivity. |
| Selected Scientific References | - Discovery of novel trimethoxy-ring BRD4 bromodomain inhibitors
- Identification of small molecule inhibitors targeting the SMARCA2 bromodomain
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