Histone H3 (23-34) K27Ac

H3K27ac is a well-established active-chromatin modification enriched at active promoters and enhancers. This short H3(23-34) peptide provides a minimal site-specific reagent for studying acetyl-lysine recognition and the biochemical contrast between K27 acetylation and methylation.

• H3K27ac binding studies
• bromodomain research
• histone acetylation assays
• chromatin-state comparisons

Acetylation neutralizes the Lys27 side-chain charge. This peptide should be compared with matched unmodified or methylated H3(23-34) forms rather than treated as equivalent to K27 methylation.

1. CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing