| Product Name | Histone H3 (23-34), Lys27 Acetylated |
| Sequence | KAAR-K(Ac)-SAPATGG |
| Mechanism & Biological Significance | H3K27ac is an active-chromatin mark enriched at active promoters and enhancers. Acetylation neutralizes the Lys27 side-chain charge and creates a chemical state that is distinct from the unmodified and methylated H3K27 forms. |
| Published Research Context | The exact KAAR-K(Ac)-SAPATGG peptide was explicitly sourced from the commercial supplier and used in a peer-reviewed study of HMGN binding to H3K27ac nucleosomes. A 1000-fold molar excess of the exact acetylated peptide was used as a competitor, helping show that the isolated acetylated H3 tail sequence alone did not account for preferential HMGN binding to acetylated nucleosomes. |
| Drug Discovery & Assay Relevance | H3K27ac competition assays |
| Research Applications | - H3K27ac competition assays
- acetyl-lysine reader studies
- chromatin-protein interaction controls
- matched comparison with unmodified H3(23-34)
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| Experimental Considerations | This is the short free-acid H3K27ac 12-mer. Do not transfer quantitative binding from longer H3K27ac peptides, biotinylated H3K27ac constructs, or intact/nucleosomal H3 without construct-specific validation. |
| Selected Scientific References | - Exact H3K27ac(23-34) peptide used in HMGN/chromatin experiments
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