Histone H3 (23-34)

Histone H3(23-34) is a compact fragment centered on Lys27, a major site of methylation and acetylation in Polycomb-associated and active chromatin. The unmodified peptide provides a reference substrate or binding control for enzymes and readers that recognize H3K27 modification states.

• H3K27 modification assays
• histone methyltransferase studies
• reader-protein controls
• epigenetic enzyme screening

This is the unmodified free-acid 12-residue H3 fragment and is the appropriate matched control for K27Ac, K27Me1, K27Me2 and K27Me3 variants.

1. Quantitative Mass Spectrometry of Histones H3.2 and H3.3 in Suz12-deficient Mouse Embryonic Stem Cells Reveals Distinct, Dynamic Post-translational Modifications at Lys-27 and Lys-36