| Product Name | Histone H3 (1-30) K14Ac/K18Ac/K23Ac/K27Ac-GGK(Biotin) |
| Product Quantity | 1 mg |
| Sequence | ARTKQTARKSTGG-K(Ac)-APR-K(Ac)-QLAT-K(Ac)-AAR-K(Ac)-SAP-GGK(Biotin) |
| Molecular Weight | 3802.4 |
| Purity | ≥95% |
| Mechanism & Biological Significance | This H3(1-30) reagent carries acetylation at Lys14, Lys18, Lys23 and Lys27 plus a C-terminal GGK(Biotin) affinity handle. It models a highly acetylated H3 tail for multivalent bromodomain and chromatin-reader studies. |
| Published Research Context | Peer-reviewed bromodomain studies directly used biotin-labeled H3(1-30) K14/18/23/27Ac peptide in lysate pull-down experiments. Independent drug-discovery profiling used H3(1-30) K14/18/23/27Ac-GG-Biotin as a bromodomain assay ligand, providing direct support for the same multi-acetylated biotinylated construct class. |
| Research Applications | - bromodomain binding assays
- streptavidin pull-down experiments
- chromatin-reader profiling
- bromodomain inhibitor screening
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| Experimental Considerations | The four acetylation sites and C-terminal biotin linker are integral to the reagent. Do not transfer results to single-acetylated H3 peptides or differently positioned biotin tags without validation. |
| Selected Scientific References | - Selective and Cell-Active PBRM1 Bromodomain Inhibitors Discovered through NMR Fragment Screening
- FT-6876, a Potent and Selective Inhibitor of CBP/p300, is Active in Preclinical Models of Androgen Receptor-Positive Breast Cancer
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