| Product Name | Histone H3 (1-25), amide |
| Product Quantity | 1 mg |
| Sequence | ARTKQTARKSTGGKAPRKQLATKAA-NH2 |
| Molecular Weight | 2625.2 |
| Purity | ≥95% |
| Mechanism & Biological Significance | Histone H3(1-25)-NH2 contains the major N-terminal H3 regulatory lysines in an amidated 25-residue sequence context and serves as an acceptor substrate for histone methyltransferase assays. |
| Published Research Context | A peer-reviewed Transcreener methyltransferase assay-development study evaluated histone-tail acceptor substrates across multiple HMT enzymes; the exact H3(1-25)-amide construct is documented as an HMT substrate and is particularly useful for G9a assay development. |
| Research Applications | - histone methyltransferase assays
- G9a/EHMT assay development
- SAH-detection methyltransferase assays
- epigenetic inhibitor screening
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| Experimental Considerations | The C-terminal amide distinguishes this construct from free-acid H3(1-25) and from shorter H3 substrates. Enzyme activity and apparent kinetic parameters can change with peptide length and terminal chemistry. |
| Selected Scientific References | - Biochemical Assay Development for Histone Methyltransferases Using a Transcreener-Based Assay for S-Adenosylhomocysteine
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