Histone H3 (1-21)-GGK(Biotin)-NH2

This construct contains the unmodified H3 N-terminal residues 1-21 followed by a GG linker and biotinylated lysine with a C-terminal amide. It provides an immobilizable reference H3 tail for enzyme, reader and histone-binding assays.

• histone binding controls
• streptavidin pull-downs
• epigenetic enzyme assays
• chromatin reader studies

The C-terminal GGK(Biotin)-NH2 handle makes this chemically distinct from free H3(1-21), free-acid GGK(Biotin) constructs and fluorescent H3 peptides.

1. Binding of the histone chaperone ASF1 to the CBP bromodomain promotes histone acetylation