| Product Name | Histone H3 (1-21)-GGK(Biotin)-NH2 |
| Product Quantity | 1 mg |
| Sequence | ARTKQTARKSTGGKAPRKQLA-GGK(Biotin)-NH2 |
| Molecular Weight | 2722.4 |
| Purity | ≥95% |
| Mechanism & Biological Significance | This construct contains the unmodified H3 N-terminal tail (residues 1-21) followed by a GG spacer and a biotinylated lysine with a terminal amide. It is an immobilizable reference ligand for comparing modification-dependent binding and enzyme recognition across the H3 tail. |
| Published Research Context | The exact the commercial supplier AS-61702 construct has been used directly in peer-reviewed peptide pull-down studies. In Legionella chromatin work, one microgram of this exact peptide was immobilized on streptavidin magnetic beads to test binding of bacterial histone-modifying proteins; the study also reported peptide purity above 95% with identity confirmed by mass spectrometry. The same catalog peptide has also been used in nuclear-protein pull-down studies involving lamin-associated chromatin regulation. |
| Research Applications | - histone peptide pull-down assays
- chromatin-reader and histone-enzyme binding
- streptavidin immobilization controls
- comparison with modified H3 tail peptides
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| Experimental Considerations | The GGK(Biotin)-NH2 handle is part of the exact reagent identity. Do not treat free H3(1-21), free-acid biotinylated H3, or differently linked biotin constructs as chemically identical. |
| Selected Scientific References | - SET and ankyrin domains of a Legionella histone methyltransferase
- SIRT7 mediates L1 transcriptional repression and nuclear-lamina association
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