| Product Name | Histone H3 (1-21) K9Me3-GGK(Biotin) |
| Product Quantity | 0.25 mg |
| Sequence | ARTKQTAR-K(Me3)-STGGKAPRKQLA-GGK(Biotin) |
| Molecular Weight | 2765.2 |
| Purity | ≥95% |
| Mechanism & Biological Significance | H3K9me3 is a canonical repressive chromatin mark recognized by HP1-family chromodomains and other methyl-lysine readers. This H3(1-21) construct combines trimethylated Lys9 with a C-terminal GGK(Biotin) capture handle, enabling direct biochemical isolation and quantification of proteins that discriminate H3K9 methylation states. |
| Published Research Context | The exact the commercial supplier AS-64360 H3(1-21) K9me3 biotinylated peptide has been used directly in peer-reviewed peptide pull-down and quantitative proteomics experiments. In fission-yeast chromatin studies, biotinylated H3K9me3 peptide from the commercial supplier was immobilized and used to identify methyl-state-dependent interacting proteins. Independent work on the putative histone demethylase Epe1 likewise lists the commercial supplier AS-64360 among the exact peptide reagents used for H3K9-methylation-dependent binding assays. |
| Research Applications | - H3K9me3 reader-protein pull-downs
- HP1/heterochromatin interaction studies
- quantitative proteomics of histone readers
- epigenetic binding and competition assays
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| Experimental Considerations | The the commercial supplier technical sheet specifies the free-acid GGK(Biotin) form. Keep this construct separate from GGK(Biotin)-NH2 or other biotin-linker variants because terminal chemistry can alter mass and assay behavior. |
| Selected Scientific References | - Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription
- An H3K9 methylation-dependent protein interaction regulates the non-enzymatic functions of a putative histone demethylase
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