Product Name | HHHHHHC, 6His-Cysteine |
Product Quantity | 1 mg |
Catalog Number | LT12030 |
Molecular Weight | 944 |
Formula | C39H49N19O8S1 |
Sequence | HHHHHHC |
Purity | 97.24% |
Product Description | The HHHHHH-Cysteine peptide (6His) is used as a fusion tag for immunoaffinity chromatography, which is consisted of six amino acids (HHHHHH). It allows elution under non-denaturing conditions. This epitope tag is a short hydrophilic, highly charged peptide. It is the most widely used epitope tag employed in structural and functional studies of proteins. Several antibodies against this peptide have been developed. |
Scientific Background | HHHHHHC, 6His-Cysteine is a synthetic research peptide in the hexahistidine (His6) affinity-tag peptide category. A six-histidine motif is a standard metal-affinity tag that coordinates immobilized transition-metal ions and is widely used in Ni2+-chelate purification and solid-phase capture. Synthetic His6-containing peptides can serve as metal-binding controls or as sequence-defined affinity-tag reagents; appended cysteine or glycine residues alter the construct without changing the core His6 motif. |
Research Applications | - Ni2+/metal-chelate affinity studies
- His-tag binding controls
- immobilized metal affinity assay development
- tag/linker sequence comparisons
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Experimental Notes | The exact supplied sequence and modification state should be used when interpreting published results. Activity reported for a parent peptide, labeled derivative, formulation, species variant, or related analog should not automatically be assigned to this construct. |
Selected References | - FLAG- and His6-tagged derivatives purified using Ni2+-chelate affinity chromatography
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