| The HHHHHH-Cysteine peptide (6His) is used as a fusion tag for immunoaffinity chromatography, which is consisted of six amino acids (HHHHHH). It allows elution under non-denaturing conditions. This epitope tag is a short hydrophilic, highly charged peptide. It is the most widely used epitope tag employed in structural and functional studies of proteins. Several antibodies against this peptide have been developed. |