HHHHHHGGG

HHHHHHGGG is a synthetic research peptide in the hexahistidine (His6) affinity-tag peptide category. A six-histidine motif is a standard metal-affinity tag that coordinates immobilized transition-metal ions and is widely used in Ni2+-chelate purification and solid-phase capture. Synthetic His6-containing peptides can serve as metal-binding controls or as sequence-defined affinity-tag reagents; appended cysteine or glycine residues alter the construct without changing the core His6 motif.

• Ni2+/metal-chelate affinity studies
• His-tag binding controls
• immobilized metal affinity assay development
• tag/linker sequence comparisons

The exact supplied sequence and modification state should be used when interpreting published results. Activity reported for a parent peptide, labeled derivative, formulation, species variant, or related analog should not automatically be assigned to this construct.

1. FLAG- and His6-tagged derivatives purified using Ni2+-chelate affinity chromatography