Generic Protease Rhodamine 110 Substrate, (Z-Arg)2Rh1102HCl

(Z-Arg)2Rh110·2HCl is a bis-arginine rhodamine 110 fluorogenic substrate for arginine-directed proteases including trypsin and lysosomal cathepsins B and L. In the intact substrate, rhodamine fluorescence is strongly suppressed. Proteolytic removal of the Z-Arg groups produces highly fluorescent rhodamine 110, providing a sensitive green readout around 501 nm excitation and 527 nm emission.

• trypsin activity assays
• cathepsin B/L activity studies
• lysosomal protease measurements
• broad protease inhibitor screening

This is a broad arginine-directed protease substrate rather than an enzyme-specific reporter. Enzyme identity in cells or lysates should be established using selective inhibitors or orthogonal assays. Protect the rhodamine reagent from light.

1. Detection of lysosomal cysteine proteinases in microglia: flow cytometric measurement and histochemical localization of cathepsin B and L
2. Trypsinogen activation in rat pancreatic acinar cells hyperstimulated by caerulein