Generic Protease Rhodamine 110 Substrate, (Z-R)2Rh1102HCl

(Z-Arg)2Rh110·2HCl is a rhodamine 110-based fluorogenic substrate for arginine-preferring proteases including trypsin and lysosomal cathepsins B and L. Proteolytic removal of the Z-Arg groups releases rhodamine 110 and produces a strong fluorescence increase, allowing sensitive measurement of protease activity in biochemical or cell-associated assays.

• trypsin activity assays
• cathepsin B/L activity measurements
• flow-cytometric protease studies
• protease inhibitor screening

The released rhodamine 110 signal is typically monitored near 501 nm excitation and 527 nm emission. This is a broad protease substrate and should not be treated as isozyme specific.

1. Detection of lysosomal cysteine proteinases in microglia: flow cytometric measurement and histochemical localization of cathepsin B and L
2. Trypsinogen activation in rat pancreatic acinar cells hyperstimulated by caerulein