Generic MMP FRET Substrate, Mca-PLGL-Dap(Dnp)-AR

Mca-PLGL-Dap(Dnp)-AR is an internally quenched fluorogenic peptide used for continuous matrix metalloproteinase assays. The Mca reporter is quenched by the Dnp group before cleavage; proteolysis within the PLGL-containing region separates fluorophore and quencher and increases fluorescence. This format is useful as a general MMP activity reagent rather than as an absolutely isoform-specific substrate.

• general MMP activity assays
• metalloproteinase inhibitor screening
• continuous fluorogenic protease assays
• comparative MMP studies

The substrate should be protected from light. Because multiple MMPs can recognize related PLGL-containing substrates, enzyme identity should be established with purified enzymes, selective inhibitors, or orthogonal controls.

1. Kinetic Analysis of Matrix Metalloproteinase Activity Using Fluorogenic Triple-Helical Substrates
2. Biochemical characterization of human collagenase-3