Catalog Number | LT8805 |
Product Name | Cathepsin D/E FRET Substrate, Mca-GKPILFFRLK(Dnp)-D-Arg-NH2 |
Product Quantity | 1 mg |
Sequence | Mca-GKPILFFRLK(Dnp)-r-NH2 |
Molecular Weight | 1756.1 |
Purity | ≥95% |
Scientific Background | Mca-GKPILFFRLK(Dnp)-D-Arg-NH2 is an internally quenched fluorogenic substrate used for the aspartic proteases cathepsin D and cathepsin E. Cleavage within the Phe-Phe region relieves quenching of the Mca reporter and increases fluorescence. The substrate has been used in tissue and cell-extract assays to quantify cathepsin D activity and in inhibitor-screening studies. |
Research Applications | - cathepsin D activity assays
- cathepsin E activity assays
- lysosomal protease research
- aspartic protease inhibitor screening
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Experimental Notes | Published applications commonly monitor the Mca signal near 328 nm excitation with emission in the 393-460 nm range depending on instrumentation. Pepstatin-class inhibitors can be useful as aspartic-protease controls. |
Selected References | - Fluorogenic substrate for cathepsins D and E
- Cathepsin D activity assay applications using Mca-GKPILFFRLK(Dnp)-D-Arg-NH2
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