Catalog Number | LT8757 |
Product Name | Caspase-3/7 Fluorogenic Substrate, Z-DEVD-AFC |
Product Quantity | 5 mg |
Sequence | Z-DEVD-AFC |
Molecular Weight | 821.7 |
Formula | C36H38F3N5O14 |
Purity | ≥95% |
Scientific Background | Z-DEVD-AFC is a fluorogenic DEVD tetrapeptide substrate used to monitor effector-caspase activity. Cleavage after the C-terminal aspartate releases AFC, producing a strong fluorescence signal. DEVD-based substrates are commonly applied to caspase-3 and caspase-7 measurements in apoptosis-related biochemical assays. |
Research Applications | - caspase-3/7 activity assays
- apoptosis research
- caspase inhibitor screening
- fluorometric protease assay development
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Experimental Notes | AFC fluorescence is typically monitored near 500 nm after excitation in the 370-390 nm range. DEVD recognition is not absolutely unique to one caspase, so enzyme controls are recommended. |
Selected References | - Fluorometric and colorimetric detection of caspase activity associated with apoptosis
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